Details

Autor(en) / Beteiligte

• Chang, Yu-Kaung
• Show, Pau-Loke
• Lan, John Chi-Wei
• Tsai, Jung-Chin
• Huang, Chi-Rong

Titel

Isolation of C-phycocyanin from Spirulina platensis microalga using Ionic liquid based aqueous two-phase system

Ist Teil von

• Bioresource technology, 2018-12, Vol.270, p.320-327

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •A simple, fast and green process for isolation of CPC was established.•CPC recovery from disrupted algae using IL-ATPS was developed.•The extraction efficiency of CPC obtained was 99%.•The partition coefficient for CPC in this study was 36.6.•The separation factor achieved in IL-ATPS was 5.8. An aqueous two-phase system (ATPS) with ionic liquids (ILs) was used for the isolate of C-phycocyanin (CPC) from Spirulina platensis microalga. Various imidazolium ILs and potassium salts were studied. The effect of ILs-ATPS on the extraction efficiency of CPC was also studied. The experimental parameters like pH, loading volume, algae concentration, temperature, and alkyl chain length of IL were well-covered in this report. The experimental results showed that the extraction efficiency, the partition coefficient, and the separation factor for CPC were 99%, 36.6, and 5.8, respectively, for an optimal pH value of 7 and a temperature of 308 K. The order of extraction efficiency for CPC using IL-ATPS was: 1-octyl-3-methylimidazolium bromide (C8MIM-Br) > 1-hexyl-3-methylimidazolium bromide (C6MIM-Br) > 1-butyl-3-methylimidazolium bromide (C4MIM-Br). The isolation process followed the pseudo second-order kinetic model and the thermodynamic results were obviously spontaneous.