Details

Autor(en) / Beteiligte

• Hwang, Eunson
• Gao, Wei
• Xiao, Yong‐kun
• Ngo, Hien T. T.
• Yi, Tae‐Hoo

Titel

Helianthus annuus L. flower prevents UVB‐induced photodamage in human dermal fibroblasts by regulating the MAPK/AP‐1, NFAT, and Nrf2 signaling pathways

Ist Teil von

• Journal of cellular biochemistry, 2019-01, Vol.120 (1), p.601-612

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2019

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Background Excessive ultraviolet radiation usually causes skin photoaging, inflammation, and even photocarcinogenesis. UV radiation‐generated reactive oxygen species (ROS) are a major contributing factor to photodamage. The flowers of Helianthus annuus L. have been reported to possess strong anti‐inflammatory and antioxidant activity. However, there are few reports on the use of H. annuus L. to relieve UVB‐induced photoaging. Purpose In this study, we evaluated the protective effect of a 50% ethanol extract of H. annuus L. flower (HAF) against UVB‐induced photodamage using normal human dermal fibroblasts. Methods The secretion of ROS, interleukin‐6 (IL‐6), vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMPs), procollagen type I, and transforming growth factor‐β1 (TGF‐β1) was measured with kits. The messenger RNA levels of COX‐2, iNOS, and TGF‐α were measured by RT‐PCR. The AP‐1, MAPK, NFAT, and Nrf2 pathways were investigated by Western blot analysis. Results HAF extract significantly blocked UVB‐induced ROS and MMP (MMP‐1 and MMP‐3) production and procollagen type I reduction. Further study demonstrated that the photoaging inhibitory actions were related to promotion of Nrf2 nuclear translocation, upregulation of TGF‐β1 level, and downregulation of AP‐1 and MAPK phosphorylation. Importantly, HAF effectively inhibited UVB‐induced VEGF and inflammatory cytokines such as IL‐6, COX‐2, iNOS, and TNF‐α secretion, which might be involved in the regulation of the NFAT signaling pathway. Conclusion Our results indicate that HAF is a useful botanical source protecting against UVB‐mediated skin photodamage. In this study, 50% ethanol extract of Helianthus annuus L. flower (HAF) significantly blocked UVB‐induced ROS and MMP (MMP‐1 and MMP‐3) production and procollagen type I reduction. It also promoted Nrf2 nuclear translocation, upregulation of TGF‐β1 level, and downregulation of AP‐1 and MAPK phosphorylation. HAF effectively inhibited UVB‐induced VEGF and inflammatory cytokines such as IL‐6, COX‐2, iNOS, and TNF‐α secretion, which might be involved in the regulation of the NFAT signaling pathway.