Details

Autor(en) / Beteiligte

• Zhu, Yingdi
• Jović, Milica
• Lesch, Andreas
• Tissières Lovey, Lysiane
• Prudent, Michel
• Pick, Horst
• Girault, Hubert H.

Titel

Immuno‐affinity Amperometric Detection of Bacterial Infections

Ist Teil von

• Angewandte Chemie International Edition, 2018-11, Vol.57 (45), p.14942-14946

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A combination of an immuno‐affinity enrichment strategy and sensitive amperometric read‐out was implemented in a point‐of‐care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices through immuno‐affinity, were detected by amperometric monitoring of the redox state of metabolic activity indicators, providing species identification and viable‐cell quantification. The method was successfully employed for the diagnosis of bacterial infections including antimicrobial susceptibility testing with only several hours of total working time. Sensing resistance: A sensitive immuno‐affinity‐based electrochemical method was proposed for bacterial pathogen identification, quantification, and antimicrobial susceptibility testing through the amperometric monitoring of the redox state of metabolic activity indicators. Using this method, the diagnosis of bacterial infections was accomplished within several hours.