Details

Autor(en) / Beteiligte

• Zhai, You-gang
• Wang, Huan-Yu
• Sun, Xiao-hong
• Fu, Shi-hong
• Wang, Huan-qin
• Attoui, Houssam
• Tang, Qing
• Liang, Guo-dong

Titel

Complete sequence characterization of isolates of Getah virus (genus Alphavirus, family Togaviridae) from China

Ist Teil von

• Journal of general virology, 2008-06, Vol.89 (6), p.1446-1456

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin St, Beijing 100052, PR China 2 Department of Arbovirology, Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, UK Correspondence Guo-dong Liang gdliang{at}hotmail.com Ten virus isolates belonging to species Getah virus (GETV) have been obtained during surveys for arboviruses in China since 1964. Seven of these isolates (YN0540, YN0542, SH05-6, SH05-15, SH05-16, SH05-17 and GS10-2) were obtained during the current study. The full-length sequences of three Chinese isolates (M1, isolated in 1964; HB0234, isolated in 2002; YN0540, isolated in 2005) were determined. The full-length sequences of these isolates were respectively 11 696, 11 686 and 11 690 nt, and showed more than 97 % intraspecies identity. Deletions were found in the capsid protein of strain M1 and non-structural protein nsP3 of strain HB0234. The E2 gene and 3' UTR of all ten isolates were also characterized. The E2 gene of the Chinese GETV isolates showed nucleotide sequence identities of 98–100 % when compared with other GETV isolates. In the 3' UTR of the Chinese isolates, an insertion of 10 consecutive adenine residues (nt 189–198) appeared in strain M1, and 9 or 3 consecutive adenines were found towards the 3' end of the third RES in strains SH05-6 and SH05-15, respectively. The 3' UTRs of the Chinese isolates showed a deletion between positions 45 and 54 and nucleotide transitions at positions 43, 64 and 148. Sequence and phylogenetic analyses showed that there was a relatively high degree of conservation among GETV isolates. The isolation of GETV from various provinces in China and also in Russia and Mongolia (including regions of the northern tundra) are an indication of changes in the world distribution of this re-emerging virus. The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are EU015061–EU015076. Details of primers used for RT-PCR and sequencing and alignments of GETV and SAGV E2 and 3' UTR sequences are available as supplementary material with the online version of this paper.