Details

Autor(en) / Beteiligte

• Seibert, C S
• Tanaka-Azevedo, A M
• Santoro, M L
• Mackessy, S P
• Soares Torquato, RJ
• Lebrun, I
• Tanaka, A S
• Sano-Martins, I S

Titel

Purification of a phospholipase A sub(2) from Lonomia obliqua caterpillar bristle extract

Ist Teil von

• Biochemical and biophysical research communications, 2006-04, Vol.342 (4), p.1027-1033

Erscheinungsjahr

2006

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Lonomia obliqua caterpillar bristle extract induces both direct and indirect hemolytic activity on human and rat washed erythrocytes, and provokes intravascular hemolysis in Wistar rats. Indirect hemolytic activity is assumed to be caused by a phospholipase A sub(2) (PLA sub(2)) present in this extract, and this investigation was initiated in order to characterize this enzyme. Phospholipase A sub(2) activity of crude extract was inhibited by both a PLA sub(2)-specific inhibitor (pBpb) and the metal ion chelator EDTA. L. obliqua PLA sub(2) was purified by liquid chromatography from the crude bristle extract and had a molecular mass of 15kDa and a pI of 5.9; its N-terminal sequence showed high homology to a sequence of a putative PLA sub(2) obtained from a cDNA library of L. obliqua bristles, and it is tentatively placed among Group III phospholipases A sub(2). This enzyme was stable at 4 super(o)C, sensitive to higher temperatures, and its maximum catalytic activity was at pH 8.0. L. obliqua PLA sub(2) induced hemolysis only when incubated with exogenous lecithin. Thus, the PLA sub(2) purified herein appears to be responsible for the indirect hemolytic activity of the crude bristle extract.