Details

Autor(en) / Beteiligte

• Vetchinova, A. S.
• Simonova, V. V.
• Novosadova, E. V.
• Manuilova, E. S.
• Nenasheva, V. V.
• Tarantul, V. Z.
• Grivennikov, I. A.
• Khaspekov, L. G.
• Illarioshkin, S. N.

Titel

Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson’s Disease

Ist Teil von

• Bulletin of experimental biology and medicine, 2018-07, Vol.165 (3), p.378-381

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2018

Quelle

Springer Online Journals【Remote access available】

Beschreibungen/Notizen

• We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.