Details

Autor(en) / Beteiligte

• Le Borgne, Marie
• Etchart, Nathalie
• Goubier, Anne
• Lira, Sergio A
• Sirard, Jean Claude
• Van Rooijen, Nico
• Caux, Christophe
• Ait-Yahia, Smina
• Vicari, Alain
• Kaiserlian, Dominique
• Dubois, Bertrand

Titel

Dendritic Cells Rapidly Recruited into Epithelial Tissues via CCR6/CCL20 Are Responsible for CD8 super(+) T Cell Crosspriming In Vivo

Ist Teil von

• Immunity (Cambridge, Mass.), 2006-02, Vol.24 (2), p.191-201

Erscheinungsjahr

2006

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• The nature of dendritic cell(s) (DC[s]) that conditions efficient in vivo priming of CD8 super(+) CTL after immunization via epithelial tissues remains largely unknown. Here, we show that myeloid DCs rapidly recruited by adjuvants into the buccal mucosa or skin are essential for CD8 super(+) T cell crosspriming. Recruitment of circulating DC precursors, including Gr1 super(+) monocytes, precedes the sequential accumulation of CD11c super(+) MHC class II super(+) DCs in dermis and epithelium via a CCR6/CCL20-dependent mechanism. Remarkably, a defect in CCR6, local neutralization of CCL20, or depletion of monocytes prevents in vivo priming of CD8 super(+) CTL against an innocuous protein antigen administered with adjuvant. In addition, transfer of CCR6-sufficient Gr1 super(+) monocytes restores CD8 super(+) T cell priming in CCR6 super( degree / degree ) mice via a direct Ag presentation mechanism. Thus, newly recruited DCs likely derived from circulating monocytes are responsible for efficient crosspriming of CD8 super(+) CTL after mucosal or skin immunization.