Details

Autor(en) / Beteiligte

• Geisenberger, Otto
• Ammendola, Aldo
• Christensen, Bjarke B
• Molin, Søren
• Schleifer, Karl-Heinz
• Eberl, Leo

Titel

Monitoring the conjugal transfer of plasmid RP4 in activated sludge and in situ identification of the transconjugants

Ist Teil von

• FEMS microbiology letters, 1999-05, Vol.174 (1), p.9-17

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

1999

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• A GFPmut3b-tagged derivative of broad host-range plasmid RP4 was used to monitor the conjugative transfer of the plasmid from a Pseudomonas putida donor strain to indigenous bacteria in activated sludge. Transfer frequencies were determined to be in the range of 4×10 −6 to 1×10 −5 transconjugants per recipient. In situ hybridisation with fluorescently labeled, rRNA-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid.