Details

Autor(en) / Beteiligte

• Lee, Lawrence Cho‐Cheung
• Cheung, Hugo Man‐Hin
• Liu, Hua‐Wei
• Lo, Kenneth Kam‐Wing

Titel

Exploitation of Environment‐Sensitive Luminophores in the Design of Sydnone‐Based Bioorthogonal Imaging Reagents

Ist Teil von

• Chemistry : a European journal, 2018-09, Vol.24 (53), p.14064-14068

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2018

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Although the strain‐promoted sydnone–alkyne cycloaddition reaction has been utilized for bioconjugation, its potential applications in bioorthogonal labeling and imaging in live cells have not been explored. This communication reports novel bioorthogonal imaging reagents with environment‐sensitive emission properties through the modification of sydnone with cyclometalated iridium(III) polypyridine complexes. These complexes displayed significant emission enhancement and lifetime elongation upon reaction with strained alkyne derivatives, and were utilized to label cyclooctyne‐modified proteins and ceramide molecules in live cells. Additionally, the manipulation of the photocytotoxicity of the complexes through the use of a bioorthogonal reagent was demonstrated. Novel bioorthogonal imaging reagents with environment‐sensitive emission properties were developed through the modification of sydnone with cyclometalated iridium(III) polypyridine complexes.