Details

Autor(en) / Beteiligte

• Taher, Yousef A
• van Esch, Betty CAM
• Hofman, Gerard A
• Henricks, Paul AJ
• van Oosterhout, Antoon JM

Titel

1 alpha ,25-Dihydroxyvitamin D sub(3) Potentiates the Beneficial Effects of Allergen Immunotherapy in a Mouse Model of Allergic Asthma: Role for IL-10 and TGF- beta

Ist Teil von

• The Journal of immunology (1950), 2008-04, Vol.180 (8), p.5211-5221

Erscheinungsjahr

2008

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• 1 alpha ,25-Dihydroxyvitamin D sub(3) (1,25(OH) sub(2)D sub(3)), a potent inhibitor of NF- Kappa B expression, can prevent the maturation of dendritic cells in vitro leading to tolerogenic dendritic cells with increased potential to induce regulatory T cells. Herein, we investigated whether the combination of allergen immunotherapy with 1,25(OH) sub(2)D sub(3) potentiates the suppressive effects of immunotherapy and whether the immunoregulatory cytokines IL-10 and TGF- beta are involved in the effector phase. OVA-sensitized and challenged BALB/c mice displayed airway hyperresponsiveness (AHR) and increased serum OVA-specific IgE levels, bronchoalveolar lavage eosinophilia, and Th2 cytokine levels. In this model, the dose response of allergen immunotherapy 10 days before OVA inhalation challenge shows strong suppression of asthma manifestations at 1 mg of OVA, but partial suppression of bronchoalveolar lavage eosinophilia, IgE up-regulation, and no reduction of AHR at 100 mu g. Interestingly, coadministration of 10 ng of 1,25(OH) sub(2)D sub(3) with 100 mu g of OVA immunotherapy significantly inhibited AHR and potentiated the reduction of serum OVA-specific IgE levels, airway eosinophilia, and Th2-related cytokines concomitant with increased IL-10 levels in lung tissues and TGF- beta and OVA-specific IgA levels in serum. Similar effects on suboptimal immunotherapy were observed by inhibition of the NF- Kappa B pathway using the selective I Kappa B kinase 2 inhibitor PS-1145. The suppressive effects of this combined immunotherapy were partially reversed by treatment with mAb to either IL-10R or TGF- beta before OVA inhalation challenge but completely abrogated when both Abs were given. These data demonstrate that 1,25(OH) sub(2)D sub(3) potentiates the efficacy of immunotherapy and that the regulatory cytokines IL-10 and TGF- beta play a crucial role in the effector phase of this mouse model.