Details

Autor(en) / Beteiligte

• Wang, Mingming
• Wang, He
• Feng, Yinghui
• Xu, Qinrui
• Admassu, Habtamu
• Yang, Ruijin
• Hua, Xiao

Titel

Preparation and Characterization of Sugar-Assisted Cross-Linked Enzyme Aggregates (CLEAs) of Recombinant Cellobiose 2‑epimerase from Caldicellulosiruptor saccharolyticus (CsCE)

Ist Teil von

• Journal of agricultural and food chemistry, 2018-07, Vol.66 (29), p.7712-7721

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• High-efficiency lactulose-producing enzyme of Caldicellulosiruptor saccharolyticus cellobiose 2-epimerase (WT-CsCE) was immobilized in the form of cross-linked enzyme aggregates (CLEAs). Conditions for enzyme aggregation and cross-linking were optimized, and a sugar-assisted strategy with less damage to enzyme secondary structures was developed to improve the activity yield of CLEAs up to approximately 65%. The resulting CLEAs with multiple-layer network structures exhibited an enlarged optimal temperature range (70–80 °C) and maintained higher activity at 50–90 °C. Besides, CLEAs retained more than 95% of their initial activity after 10 successive batches at 60 °C, demonstrating superior reusability. Moreover, CLEAs displayed an equivalent or higher catalytic ability to free WT-CsCE in lactulose biosynthesis, and the final sugar ratios were similar, lactulose 58.8–61.7%, epilactose 9.3–10.2%, and lactose 27.8–30%, with a constant isomerization selectivity of 0.84–0.87 regardless of enzymes used and temperature applied. The proposed strategy is the first trial for enzymatic synthesis of lactulose catalyzed by CLEAs of WT-CsCE.