Details

Autor(en) / Beteiligte

• Ezoulin, Miezan J.M.
• Ombetta, Jean-Edouard
• Dutertre-Catella, Hélène
• Warnet, Jean-Michel
• Massicot, France

Titel

Antioxidative properties of galantamine on neuronal damage induced by hydrogen peroxide in SK–N–SH cells

Ist Teil von

• Neurotoxicology (Park Forest South), 2008-03, Vol.29 (2), p.270-277

Ort / Verlag

Orlando, FL: Elsevier B.V

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• Galantamine, an acetylcholinesterase inhibitor used to enhance memory in AD patients by acetylcholinesterase inhibition, has been tested for its protective properties on an in vitro model of H 2O 2-induced oxidative stress. SK–N–SH cells treated with H 2O 2 for 2 h showed an increase in ROS production (54%) and in NO production (52%) together with a marked reduction of the mitochondrial membrane potential (19%). These features, typical of the oxidative injury that accompanies AD, were partly recovered by galantamine. Galantamine reduced the release of reactive oxygen species (up to 50%) and prevented loss in mitochondrial activity. When SK–N–SH cells were treated with H 2O 2 for 24 h, nitrite generation was increased by twice compared with 2 h. Galantamine treatment resulted in a significant inhibition of H 2O 2-induced nitrite generation whatever the concentration tested with a return to control values. Galantamine also concentration-dependently inhibited AChE activity (28–88%) in H 2O 2–SK–N–SH cells after 24 h. This drug, which facilitates cholinergic neurotransmission, is also neuroprotective by lowering oxidative injury. Our study provides a better understanding of the mechanisms of protection of this acetylcholinesterase inhibitor which also has antioxidative properties.