Details

Autor(en) / Beteiligte

• Kang, Ka-Won
• Jung, Jik-Han
• Hur, Woojune
• Park, Jaena
• Shin, Hyunku
• Choi, Byeonghyeon
• Jeong, Hyesun
• Kim, Dae Sik
• Yu, Eun Sang
• Lee, Se Ryeon
• Sung, Hwa Jung
• Kim, Seok Jin
• Choi, Chul Won
• Kim, Hyun Koo
• Hong, Sunghoi
• Park, Ji-Ho
• Choi, Yeonho
• Park, Yong
• Kim, Byung Soo

Titel

The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker

Ist Teil von

• Anticancer research, 2018-07, Vol.38 (7), p.3935-3942

Ort / Verlag

Greece: International Institute of Anticancer Research

Erscheinungsjahr

2018

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities. Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick™ exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript. Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This ~250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript. CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.