Details

Autor(en) / Beteiligte

• Mitsumasu, K.(Nagoya Univ. (Japan))
• Niimi, T
• Yamashita, O
• Yaginuma, T

Titel

Expression of mRNAs for trehalase-1 and -2 in brain-subesophageal ganglion during pupal-adult development of the silkworm, Bombyx mori

Ist Teil von

• Journal of Insect Biotechnology and Sericology, 2008, Vol.77(3), pp.3_151-3_157

Ort / Verlag

Ibaraki: The Japanese Society of Sericultural Science

Erscheinungsjahr

2008

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• First, we confirmed that a trehalase specific inhibitor, validoxylamine A, injected into diapause-egg producing pupae produced significant numbers of nondiapause eggs, suggesting that biosynthesis of diapause hormone and/or its release into the hemolymph may be reduced by inhibition of trehalase activity in brain-subesophageal ganglion (SG). Next, to better understand trehalose catabolic activities in brain-SG of diapause- and nondiapause-egg producers of the silkworm, Bombyx mori, we examined the expression of trehalase-1 and -2 mRNAs during pupal-adult development. These mRNAs code for soluble and integral-membrane proteins, respectively. Remarkable differences in the mRNA amounts were not found in either the diapause- and nondiapause-egg producers, but the expression of these mRNAs increased much more rapidly prior to adult emergence, as compared with dopa decarboxylase mRNA, a key enzyme in the formation of the neurotransmitter dopamine. These results suggest involvement of trehalases within brain-SG in adult ecdysis.