Details

Autor(en) / Beteiligte

• Zhou, Wenhua
• Cui, Haodong
• Ying, Liming
• Yu, Xue‐Feng

Titel

Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing

Ist Teil von

• Angewandte Chemie International Edition, 2018-08, Vol.57 (32), p.10268-10272

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• A biodegradable two‐dimensional (2D) delivery platform based on loading black phosphorus nanosheets (BPs) with Cas9 ribonucleoprotein engineered with three nuclear localization signals (NLSs) at C terminus (Cas9N3) is successfully established. The Cas9N3‐BPs enter cells effectively via membrane penetration and endocytosis pathways, followed by a BPs biodegradation‐associated endosomal escape and cytosolic releases of the loaded Cas9N3 complexes. The Cas9N3‐BPs thus provide efficient genome editing and gene silencing in vitro and in vivo at a relatively low dose as compared with other nanoparticle‐based delivery platforms. This biodegradable 2D delivery platform offers a versatile cytosolic delivery approach for CRISPR/Cas9 ribonucleoprotein and other bioactive macromolecules for biomedical applications. Cas9 in black: A biodegradable two‐dimensional delivery platform for CRISPR/Cas9 is successfully established. It is based on loading black phosphorus nanosheets with engineered Cas9 ribonucleoprotein, enabling controlled cytosolic release and genome editing with high efficiency.