Details

Autor(en) / Beteiligte

• Zheng, Chuanlin
• Acheampong, Atiako Kwame
• Shi, Zhaowan
• Mugzech, Amichay
• Halaly‐Basha, Tamar
• Shaya, Felix
• Sun, Yufei
• Colova, Violeta
• Mosquna, Assaf
• Ophir, Ron
• Galbraith, David W.
• Or, Etti

Titel

Abscisic acid catabolism enhances dormancy release of grapevine buds

Ist Teil von

• Plant, cell and environment, 2018-10, Vol.41 (10), p.2490-2503

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2018

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• The molecular mechanism regulating dormancy release in grapevine buds is as yet unclear. It was formerly proposed that dormancy is maintained by abscisic acid (ABA)‐mediated repression of bud–meristem activity and that removal of this repression triggers dormancy release. It was also proposed that such removal of repression may be achieved via natural or artificial up‐regulation of VvA8H‐CYP707A4, which encodes ABA 8′‐hydroxylase, and is the most highly expressed paralog in grapevine buds. The current study further examines these assumptions, and its experiments reveal that (a) hypoxia and ethylene, stimuli of bud dormancy release, enhance expression of VvA8H‐CYP707A4 within grape buds, (b) the VvA8H‐CYP707A4 protein accumulates during the natural transition to the dormancy release stage, and (c) transgenic vines overexpressing VvA8H‐CYP707A4 exhibit increased ABA catabolism and significant enhancement of bud break in controlled and natural environments and longer basal summer laterals. The results suggest that VvA8H‐CYP707A4 functions as an ABA degrading enzyme, and are consistent with a model in which the VvA8H‐CYP707A4 level in the bud is up‐regulated by natural and artificial bud break stimuli, which leads to increased ABA degradation capacity, removal of endogenous ABA‐mediated repression, and enhanced regrowth. Interestingly, it also hints at sharing of regulatory steps between latent and lateral bud outgrowth. It was formerly proposed that removal of ABA‐mediated repression is required for grape bud dormancy release and may be achieved via up‐regulation of the bud‐expressed ABA 8′‐hydroxylase (VvA8H‐CYP707A4). Here, we support this proposition, showing that artificial and natural stimuli of dormancy release up‐regulates VvA8H‐CYP707A4 transcript and protein level, and its overexpression in transgenic vines significantly increased ABA catabolism and enhanced bud break.