Details

Autor(en) / Beteiligte

• Molina-Miras, A.
• López-Rosales, L.
• Sánchez-Mirón, A.
• Cerón-García, M.C.
• Seoane-Parra, S.
• García-Camacho, F.
• Molina-Grima, E.

Titel

Long-term culture of the marine dinoflagellate microalga Amphidinium carterae in an indoor LED-lighted raceway photobioreactor: Production of carotenoids and fatty acids

Ist Teil von

• Bioresource technology, 2018-10, Vol.265, p.257-267

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2018

Quelle

ScienceDirect

Beschreibungen/Notizen

• [Display omitted] •First ever long-term culture of a dinoflagellate microalga in a raceway PBR.•Multispectral light-emission diodes (LEDs) used with a raceway photobioreactor.•Sustainable supply of dinoflagellate biomass in uniform quality and yield.•Amphidinium carterae produced significant amounts of peridinin and PUFAs. The feasibility of the long-term (>170 days) culture of a dinoflagellate microalga in a raceway photobioreactor is demonstrated for the first time. Amphidinium carterae was chosen for this study as it is producer of interesting high-value compounds. Repeated semicontinuous culture provided to be a robust operational mode. Different concentration levels of the f/2 medium nutrients (i.e. f/2×1–3) were assayed. The composition f/2×3 (N:P = 5), combined with a sinusoidal irradiance pattern (L/D = 24:0) with a 570 µE m−2 s−1 daily mean irradiance, maximized the biomass productivity (2.5 g m−2 day−1) and production rate of the valuable carotenoid peridinin (19.4 ± 1.35 mg m−2 L−1 with nearly 1% of the biomass d.w.). Several carotenoids and polyunsaturated fatty acids were also present in significant percentages in the harvested biomass (EPA, 1.69 ± 0.31% d.w.; DHA, 3.47 ± 0.24% d.w.), which had an average P-molar formulate of C40.7O21.2H73.9N3.9S0.3P1.