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Application of tissue‐engineered pericardial patch in rat models of myocardial infarction
Journal of biomedical materials research. Part A, 2018-10, Vol.106 (10), p.2670-2678
Kameli, Seyedeh Maryam
Khorramirouz, Reza
Eftekharzadeh, Sahar
Fendereski, Kiarad
Daryabari, Seyedeh Sima
Tavangar, Seyed Mohammad
Kajbafzadeh, Abdol‐Mohammad
2018
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Kameli, Seyedeh Maryam
Khorramirouz, Reza
Eftekharzadeh, Sahar
Fendereski, Kiarad
Daryabari, Seyedeh Sima
Tavangar, Seyed Mohammad
Kajbafzadeh, Abdol‐Mohammad
Titel
Application of tissue‐engineered pericardial patch in rat models of myocardial infarction
Ist Teil von
Journal of biomedical materials research. Part A, 2018-10, Vol.106 (10), p.2670-2678
Ort / Verlag
United States: Wiley Subscription Services, Inc
Erscheinungsjahr
2018
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
Myocardial infarction (MI) is a major cause of mortality and morbidity in industrialized societies. Myocardial tissue engineering is an alternative and promising approach for substituting injured myocardium through development and seeding of appropriate scaffolds. In this study, we investigated the efficacy of using an acellular pericardium to deliver autologous mesenchymal stem cells (MSCs) to the infarcted site for regeneration of the myocardium. MI was induced in two groups of rats; G1 or MI group, and G2 or patch‐implanted group. In G2 group, rats had undergone transplantation of a pericardial patch which was previously seeded with adipose tissue derived MSCs. To evaluate the efficacy of the pericardial patches, biopsies were taken one month after transplantation. In order to evaluate the extent of regeneration, inflammation and fibrosis, histopathological investigations including hematoxylin and eosin (H&E), Sirius Red and trichrome staining were performed. In addition, immunohistochemical investigations by Desmin as well as CD68, CD45 and CD34 antibodies were performed. Furthermore, Tunnel assay was performed to detect the extent of apoptosis. H&E assessments of biopsies from the patch‐implanted group confirmed presence of pre‐seeded pericardium containing MSCs along with neo‐vessels. Immunohistochemical assessments demonstrated higher number of CD34 positive cells and Desmin‐positive cells in the patch implanted group (p < 0.05); these findings are suggestive of cardiomyocyte regeneration in G2 rats. This study demonstrates the advantages of application of natural acellular scaffolds as cell delivery devices and it emphasizes neovascularization following this approach. However, further investigations are required to analyze long‐term cardiac function in recipients. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2670–2678, 2018.
Sprache
Englisch
Identifikatoren
ISSN: 1549-3296
eISSN: 1552-4965
DOI: 10.1002/jbm.a.36464
Titel-ID: cdi_proquest_miscellaneous_2055613255
Format
–
Schlagworte
Adipose tissue
,
Animal models
,
Antibodies
,
Apoptosis
,
Assessments
,
Autografts
,
Biopsy
,
Blood vessels
,
Cardiomyocytes
,
CD34 antigen
,
CD45 antigen
,
cell‐ and tissue‐based therapy
,
Desmin
,
Fibrosis
,
guided tissue regeneration
,
Heart attacks
,
Mesenchyme
,
Morbidity
,
Myocardial infarction
,
Myocardium
,
Pericardium
,
Rats
,
Regeneration
,
Rodents
,
Scaffolds
,
Stem cell transplantation
,
Stem cells
,
Tissue engineering
,
Transplantation
,
Vascularization
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