Neuroreport, 2007-02, Vol.18 (3), p.235-239
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Nucleoside transporter expression and adenosine uptake in the rat cochlea
- Ist Teil von
- Neuroreport, 2007-02, Vol.18 (3), p.235-239
- Ort / Verlag
- Hagerstown, MD: Lippincott Williams & Wilkins, Inc
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Even though extracellular adenosine plays multiple roles in the cochlea, the mechanisms that control extracellular adenosine concentrations in this organ are unclear. This study investigated the expression of nucleoside transporters and adenosine uptake in the rat cochlea. Reverse transcription-polymerase chain reaction revealed the expression of mRNA transcripts for two equilibrative (ENT1 and ENT2) and two concentrative (CNT1 and CNT2) nucleoside transporters. Exogenous adenosine perfused through the cochlear perilymphatic compartment was taken up by cells lining the compartment. Adenosine uptake was sensitive to changes in extracellular Na concentrations and inhibited by nitrobenzylthioinosine (an adenosine uptake blocker). The study suggests that the bi-directional nucleoside transport supports the uptake and recycling of purines and regulates the activation of adenosine receptors by altering adenosine concentrations in cochlear fluid spaces.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0959-4965eISSN: 1473-558XDOI: 10.1097/WNR.0b013e3280115918Titel-ID: cdi_proquest_miscellaneous_20539684
- Format
- –
- Schlagworte
- Adenosine - metabolism, Adenosine - pharmacology, Animals, Biological and medical sciences, Carrier Proteins - genetics, Cochlea - drug effects, Cochlea - metabolism, Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation, Equilibrative Nucleoside Transporter 1, Female, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation - genetics, Hair Cells, Auditory - drug effects, Hair Cells, Auditory - metabolism, Male, Membrane Transport Proteins - genetics, Nucleoside Transport Proteins - genetics, Perilymph - drug effects, Perilymph - metabolism, Rats, Rats, Wistar, RNA, Messenger - metabolism, Signal Transduction - drug effects, Signal Transduction - physiology, Sodium - metabolism, Sodium Channels - drug effects, Sodium Channels - metabolism, Thioinosine - analogs & derivatives, Thioinosine - pharmacology, Vertebrates: nervous system and sense organs