Details

Autor(en) / Beteiligte

• Volod'ko, A.V.
• Davydova, V.N.
• Nedashkovskaya, O.I.
• Terentieva, N.A.
• Chusovitin, E.A.
• Galkin, N.G.
• Yermak, I.M.

Titel

Morphology, electrokinetic characteristics and the effect on biofilm formation of carrageenan:chitosan polyelectrolyte complexes

Ist Teil von

• International journal of biological macromolecules, 2018-10, Vol.117, p.1118-1124

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Carrageenan:chitosan (CG:CH) polyelectrolyte complexes (PEC) were obtained and the effect of the initial components ratio on formation was studied by dynamic light scattering, atomic force microscopy (AFM) and electrokinetic measurements. Positively charged PEC particles (average ζ-potential 40.2 mV) were formed, provided that the polycation was present in excess in the complex and was stabilized by chitosan amino groups. According to the AFM data, chitosan was located on the surface of the carrageenan fibers. In PEC where carrageenan prevailed, the number of unbound sulfate groups decreased when the chitosan content increased, this resulted in a decrease in the PEC negative surface charge (from −92.4 to −55.6 mV). In this case, AFM showed that chitosan was incorporated into the network structure of carrageenan and breaks it at a CG:CH ratio of 1:0.5 w/w. Complexes with a high content of kappa-CG inhibited biofilm formation by Gram negative and Gram positive microorganisms. •Positively and negative charged particles of carrageenan:chitosan complexes were obtained.•Chitosan was located on the surface of the carrageenan fibers in positive charged complexes.•Chitosan incorporated into the network structure of carrageenan in negative charged complexes.•Complexes with a high content of carrageenan inhibited biofilm formation by microorganisms.