Effects of hypoxia-inducible factor 1α on hypoxic tolerance of human amniotic mesenchymal stem cells
2018
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Effects of hypoxia-inducible factor 1α on hypoxic tolerance of human amniotic mesenchymal stem cells
- Ist Teil von
- Zhongguo xiu fu chong jian wai ke za zhi, 2018-03, Vol.32 (3), p.264
- Ort / Verlag
- China
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Under hypoxic conditions, the survival and apoptosis of human amniotic mesenchymal stem cells (hAMSCs) were observed by transient transfection of hypoxia-inducible factor 1α (HIF-1α) gene, to investigate the effect of HIF-1α on hypoxic tolerance of hAMSCs. The hAMSCs were isolated and cultured from amniotic membrane tissue from voluntary donors who were treated with cesarean section. And the morphological observation by inverted phase contrast microscope and immunofluorescence detection of the expressions of stem cell markers OCT-4 and NANOG were performed to identify the cultured cells. The third generation hAMSCs were treated with 200 μmol/L CoCl , and transient transfection of plasmids were added according to the following grouping: group A was hAMSCs blank group; group B was pcDNA3.1 negative control group; group C was short hairpin RNA (shRNA) negative control group; group D was shRNA-HIF-1α interference group; group E was pcDNA3.1-HIF-1α over expression group. Cell survival rate of each group was measur
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1002-1892DOI: 10.7507/1002-1892.201710104Titel-ID: cdi_proquest_miscellaneous_2046013207
- Format
- –
- Schlagworte
- Amnion - metabolism, Apoptosis, Caspase 3, Cell Hypoxia, Cell Survival, Cells, Cultured, Female, Humans, Hypoxia-Inducible Factor 1, alpha Subunit - metabolism, Mesenchymal Stem Cells, Plasmids - genetics, Pregnancy, RNA, Small Interfering, Transfection, Vascular Endothelial Growth Factor A - metabolism