Details

Autor(en) / Beteiligte

• Borgonovo, Zaine L.M.
• Ribeiro, Caroline F.
• Costa, Michele D.M.
• Souza, Ingrid L.M.
• Rossi, Gustavo R.
• Alcantara, Monica V.
• Ingberman, Max
• Braga, Luciano G.
• Mercadante, Adriana F.
• Nakao, Lia S.
• Zanata, Silvio M.

Titel

Monoclonal Antibody DL11C8 Identifies ADAM23 as a Component of Lipid Raft Microdomains

Ist Teil von

• Neuroscience, 2018-08, Vol.384, p.165-177

Ort / Verlag

United States: Elsevier Ltd

Erscheinungsjahr

2018

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• •Monoclonal antibody DL11C8 specifically recognizes ADAM23 from different species.•ADAM23 is highly expressed in tumoral cell lineages of the central nervous system.•Pro-ADAM23 is unconventionally present on the cell surface of neuronal cell lineages.•ADAM23 is the first non-catalytic member of the ADAM family detected in lipid rafts. A disintegrin and metalloprotease protein 23 (ADAM23) is a transmembrane type I glycoprotein involved with the development and maintenance of the nervous system, including neurite outgrowth, neuronal adhesion and differentiation and regulation of synaptic transmission. In addition, ADAM23 seems to participate in immune response and tumor establishment through interaction with different members of integrin receptors. Here, we describe a novel monoclonal antibody (DL11C8) that specifically recognizes the cysteine-rich domain of both pre-protein (100 kDa) and mature (70 kDa) forms of ADAM23 from different species, including human, rodents and avian orthologs. Using this antibody, we detected both forms of ADAM23 on the cell surface of three neuronal cell lineages (Neuro-2a, SH-SY5Y and CHLA-20), with a higher relative content of ADAM23100 kDa. Furthermore, we demonstrate for the first time that a catalytically inactive member of the ADAM family is present in the membrane signaling platforms, namely lipid rafts. Indeed, the mature ADAM2370 kDa partitions between raft and non-raft membrane domains, while the pro-protein ADAM23100 kDa is mainly expressed in non-raft domains. These membranous distributions were observed in both different brain regions homogenates and primary cultured neurons lysates from mouse cortex and cerebellum. Taken together, these findings point out ADAM23 as a lipid raft molecular component.