Details

Autor(en) / Beteiligte

• Yan, Zheng-Fei
• Lin, Pei
• Li, Chang-Tian
• Kook, MooChang
• Yi, Tae-Hoo

Titel

Scopulibacillus cellulosilyticus sp. nov., a cellulose-degrading bacterium isolated from tea

Ist Teil von

• Antonie van Leeuwenhoek, 2018-11, Vol.111 (11), p.2087-2094

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• A Gram-stain positive, aerobic, non-motile, endospore-forming and rod-shaped strain (THG-NT9 T ) was isolated from a green tea sample. Growth occurred at 20–45 °C (optimum 28–35 °C), at pH 6.0–8.0 (optimum 7.0) and at 0–2.0% NaCl (optimum 0%). Based on 16S rRNA gene sequence analysis, the near phylogenetic neighbours of strain THG-NT9 T were identified as Scopulibacillus daqui DSM 28236 T (98.6%), Scopulibacillus darangshiensis DSM 19377 T (97.4%), Pullulanibacillus pueri CGMCC 1.12777 T (96.7%) and Pullulanibacillus camelliae CGMCC 1.15371 T (96.3%). The DNA G + C content of strain THG-NT9 T was determined to be 47.5 mol %. DNA–DNA hybridization values between strain THG-NT9 T and S. daqui DSM 28236 T , S. darangshiensis DSM 19377 T , P. pueri CGMCC 1.12777 T , P. camelliae CGMCC 1.15371 T and Pullulanibacillus naganoensis DSM 10191 T were 41.3 ± 0.1 (39.4 ± 0.4% reciprocal analysis), 39.1 ± 0.1 (37.3 ± 0.1%), 21.4 ± 0.7 (20.1 ± 0.3%), 20.7 ± 0.1 (20.1 ± 0.4%) and 12.1 ± 0.2% (8.3 ± 0.2%). The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids. The quinone was identified as MK-7. The major fatty acids were C 18:3 ω 7 c , iso -C 15:0 , iso -C 16:0 , iso -C 17:0 and anteiso -C 17:0 . The cell wall type was determined to be A1γ peptidoglycan with meso -diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid and glucose as the cell wall sugar. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics, and DNA–DNA hybridization data, strain THG-NT9 T represents a novel species of the genus Scopulibacillus , for which the name Scopulibacillus cellulosilyticus sp. nov. is proposed. The type strain is THG-NT9 T (= KCTC 33918 T  = CGMCC 1.16305 T ).