Details

Autor(en) / Beteiligte

• Sedgwick, Adam C
• Han, Hai-Hao
• Gardiner, Jordan E
• Bull, Steven D
• He, Xiao-Peng
• James, Tony D

Titel

The development of a novel AND logic based fluorescence probe for the detection of peroxynitrite and GSH

Ist Teil von

• Chemical science (Cambridge), 2018-04, Vol.9 (15), p.3672-3676

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2018

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• We have developed a novel AND logic based fluorescence probe for the simultaneous detection of ONOO and GSH ( ). The probe was synthesised over three steps starting from commercially available fluorescein. The probe was constructed by attaching the GSH reactive motif, 2,4-dinitrobenzenesulfonyl, to the previously reported boronate fluorescence based probe, . produced only a small fluorescence response towards the addition of GSH or ONOO separately. However, when the probe was exposed to both analytes, there was a significant (40-fold) fluorescence enhancement. demonstrated an excellent selectivity towards both GSH and ONOO . In cellular imaging experiments the probe was shown to be cell permeable with no 'turn-on' response observed for the addition of either GSH or ONOO separately. However, in the presence of both analytes, a clear fluorescence response was observed in live cells. was further able to monitor the co-existence of metabolically produced ONOO and GSH by exogenous stimulation.