Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Ergebnis 18 von 162

Details

Autor(en) / Beteiligte
Titel
Population and community structure shifts of ammonia oxidizers after four-year successive biochar application to agricultural acidic and alkaline soils
Ist Teil von
  • The Science of the total environment, 2018-04, Vol.619-620, p.1105-1115
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
2018
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
  • Long-term studies that advance our mechanistic understanding of biochar (BC)‑nitrogen (N) interactions in agricultural soils are lacking. In this study, soil potential nitrification rates (PNR), the abundance and composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) communities following 4-year of BC application were investigated using the shaken-slurry procedure and molecular sequencing techniques for an acidic Oxisol (QU) and an alkaline Cambisol (YU). Soils were obtained from an outdoor soil column experiment with straw-BC application rates of 0 (BC0), 2.25 (BC2.25) and 11.3 (BC11.3) Mgha−1 per cropping season for eight consecutive wheat/millet seasons. Quantitative polymerase chain reaction (qPCR) and 454 high-throughput pyrosequencing techniques were performed to quantify and sequence amoA gene copies and composition of AOA and AOB. Results showed that QU had lower PNR and a higher ratio of amoA gene copies of AOA to AOB than YU, PNR of QU with BC application was significantly associated with the amoA gene of AOB. Similar to previous short-term findings, BC application enhanced QU soil nitrification, which may be explained by the significant increase in AOB abundance and a shift in AOB community structure from Nitrosospira cluster 2 toward cluster 3, along with the disappearance of some obligate acidophile AOA groups, leading to the appearance of ammonia-oxidizers from neutral-alkaline soils in BC-amended acid soils. Canonical correspondence analysis (CCA) showed that soil pH was the most important factor driving shifts in ammonia-oxidizers composition. Although BC application did not have significant effects on PNR in YU, BC11.3 decreased AOA and AOB gene copies and influenced the relative abundance of community structure. Our findings represent the first investigation of long-term BC effects on AOA and AOB communities in agricultural soils using 454 high-throughput pyrosequencing, showing that BC application can alter soil characteristics and influence ammonia oxidizer community composition, abundance, especially in acid soils. [Display omitted] •Response of ammonia-oxidizers in a 4-year outdoor soil column experiment with long-term BC-amendment was examined using 454 high-throughput pyrosequencing.•PNR of QU-soils amended with BC was significantly correlated with amoA gene abundances of AOB, but not AOA.•Long-term BC amendment stimulated nitrification in the acidic soil, with shifts in the AOB community structure from Nitrosospira cluster 2 toward cluster 3.•Some ammonia-oxidizer groups from neutral-alkaline soils appeared in long-term BC-amended acid soils.
Sprache
Englisch
Identifikatoren
ISSN: 0048-9697
eISSN: 1879-1026
DOI: 10.1016/j.scitotenv.2017.11.029
Titel-ID: cdi_proquest_miscellaneous_2036198181

Weiterführende Literatur

Empfehlungen zum selben Thema automatisch vorgeschlagen von bX