Angewandte Chemie (International ed.), 2001-11, Vol.40 (22), p.4201-4204
2001
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Details
- Autor(en) / Beteiligte
- Titel
- A High-Throughput-Screening Method for the Identification of Active and Enantioselective Hydrolases
- Ist Teil von
- Angewandte Chemie (International ed.), 2001-11, Vol.40 (22), p.4201-4204
- Ort / Verlag
- Weinheim: WILEY-VCH Verlag GmbH
- Erscheinungsjahr
- 2001
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- A rapid and reliable test for the determination of hydrolase activity and enantioselectivity comprises the conversion of acetic acid released from acetates to NADH by using a commercially available enzymatic test‐kit (see scheme). The NADH is spectrophotometrically quantified in a microtiter plate format.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1433-7851eISSN: 1521-3773DOI: 10.1002/1521-3773(20011119)40:22<4201::AID-ANIE4201>3.0.CO;2-VTitel-ID: cdi_proquest_miscellaneous_2033378782
- Format
- –
- Schlagworte
- biotransformations, enantioselectivity, enzyme catalysis, high-throughput screening, molecular evolution