Vaccine, 2007-05, Vol.25 (20), p.3946-3954
2007
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- Autor(en) / Beteiligte
- Titel
- Diminished intestinal colonization by Clostridium difficile and immune response in mice after mucosal immunization with surface proteins of Clostridium difficile
- Ist Teil von
- Vaccine, 2007-05, Vol.25 (20), p.3946-3954
- Ort / Verlag
- Oxford: Elsevier Ltd
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Clostridium difficile pathogenesis is mainly due to toxins A and B. However, the first step of pathogenesis is the colonization process. We evaluated C. difficile surface proteins as vaccine antigens to diminish intestinal colonization in a human flora-associated mouse model. First, we used the flagellar cap protein FliD of C. difficile , in order to test several immunization routes: intranasal, rectal, and intragastric. The rectal route, which is the most efficient, was used to vaccine groups of mice with different antigen combinations. After immunizations, the mice were challenged with the toxigenic C. difficile and a significant statistical difference between the control group and the immunized groups was observed in the colonization levels of C. difficile.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2007.02.055Titel-ID: cdi_proquest_miscellaneous_20279112
- Format
- –
- Schlagworte
- Administration, Rectal, Allergy and Immunology, Animals, Antibodies, Bacterial - biosynthesis, Antibodies, Bacterial - immunology, Applied microbiology, Bacterial Outer Membrane Proteins - chemistry, Bacterial Outer Membrane Proteins - immunology, Bacterial Proteins - chemistry, Bacterial Proteins - immunology, Bacterial Vaccines - administration & dosage, Bacterial Vaccines - immunology, Biological and medical sciences, Cholera Toxin - immunology, Cholera Toxin - pharmacology, Clostridium difficile, Clostridium difficile - growth & development, Clostridium difficile - immunology, Cysteine Endopeptidases - chemistry, Cysteine Endopeptidases - immunology, Enterocolitis, Pseudomembranous - immunology, Enterocolitis, Pseudomembranous - microbiology, Female, Fundamental and applied biological sciences. Psychology, Humans, Immunity, Mucosal - immunology, Immunization, Immunoglobulin A - biosynthesis, Immunoglobulin A - immunology, Intestines - immunology, Intestines - microbiology, Mice, Mice, Inbred C3H, Microbiology, Surface proteins, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)