Details

Autor(en) / Beteiligte

• Tsanev, R.
• Boyanova, M.
• Ivanov, I.

Titel

Fluorescence Analysis of Interferon-Gamma

Ist Teil von

• Biotechnology, biotechnological equipment, 2007, Vol.21 (2), p.215-219

Ort / Verlag

Taylor & Francis

Erscheinungsjahr

2007

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Equations were derived describing the fluorescence intensity of IFNy solutions on the basis of a dimer-monomers equilibrium of the native protein. The experimental data were found to fit very well the theoretical values predicted by these equations. Thus they open the possibility of studying the effect of different factors on parameters reflecting the conformational state and the biological activity of IFNy. The dimer-monomers dissociation upon dilution of the interferon solution opens the possibility to assess its native state. The presence and the size of a fraction unable to dissociate and to form active dimers (aggregates due to denaturation or covalent dimers due to glycation) can be revealed by the relative fluorescence decrease upon dilution of the hIFNy solution ("dilution test").