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Glucoamylase production has been investigated by solid-state fermentation of agro-industrial wastes generated during the processing of paddy to rice flakes (categorized as coarse, medium and fine waste), along with wheat bran and rice powder by a local soil isolate
Aspergillus sp. HA-2. Highest enzyme production was obtained with wheat bran (264
±
0.64
U/gds) followed by coarse waste (211.5
±
1.44
U/gds) and medium waste (192.1
±
1.15
U/gds) using 10
6
spores/ml as inoculum at 28
±
2
°C, pH 5. A combination of wheat bran and coarse waste (1:1) gave enzyme yield as compared to wheat bran alone. Media supplementation with carbon source (0.04
g/gds) as sucrose in wheat bran and glucose in coarse and medium waste increased enzyme production to 271.2
±
0.92, 220.2
±
0.75 and 208.2
±
1.99
U/gds respectively. Organic nitrogen supplementation (yeast extract and peptone, 0.02
g/gds) showed a higher enzyme production compared to inorganic source. Optimum enzyme activity was observed at 55
°C, pH 5. Enzyme activity was enhanced in the presence of calcium whereas presence of EDTA gave reverse effect.