Details

Autor(en) / Beteiligte

• Kayama, Shizuo
• Yano, Raita
• Yamasaki, Katsutoshi
• Fukuda, Chiemi
• Nishimura, Keiko
• Miyamoto, Hitoshi
• Ohge, Hiroki
• Sugai, Motoyuki

Titel

Rapid identification of carbapenemase-type blaGES and ESBL-type blaGES using multiplex PCR

Ist Teil von

• Journal of microbiological methods, 2018-05, Vol.148, p.117-119

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Guiana extended-spectrum (GES) β-lactamases are emerging in Japan. The GES family can be classified into 2 groups, one with extended-spectrum β-lactamase (ESBL)-like activity, which hydrolyzes penicillins and cephalosporins, and the other with carbapenemase-like activity with an extended spectrum toward carbapenems. This difference is mediated by variations in a specific amino acid in the GES protein: G170 N or G170S substitutions. We developed an amplification refractory mutation system (ARMS) PCR assay that enabled rapid identification of these variant genes without sequencing. •We developed a multiplex PCR to find out carbapenemase-type blaGES group among blaGES gene family.•The difference between the two amplicons is 100 bp, permitting easy visual differentiation between the PCR products.•For this PCR detection, rTaq polymerase was the best to amplify the two amplicon.