Details

Autor(en) / Beteiligte

• Lin, Pei
• Yan, Zheng-Fei
• Li, Chang-Tian
• Kook, MooChang
• Yi, Tae-Hoo

Titel

Nibribacter flagellatus sp. nov., isolated from rhizosphere of Hibiscus syriacus and emended description of the genus Nibribacter

Ist Teil von

• Antonie van Leeuwenhoek, 2018-10, Vol.111 (10), p.1777-1784

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• A Gram-stain negative, aerobic, motile by flagella, rod-shaped strain (THG-T16 T ) was isolated from rhizosphere of Hibiscus syriacus . Growth occurred at 10–40 °C (optimum 28–30 °C), at pH 6.0–8.0 (optimum 7.0) and at 0–1.0% NaCl (optimum 0%). Based on 16S rRNA gene sequence analysis, the near phylogenetic neighbours of strain THG-T16 T were identified as Nibribacter koreensis KACC 16450 T (98.6%), Rufibacter roseus KCTC 42217 T (94.7%), Rufibacter immobilis CCTCC AB 2013351 T (94.5%) and Rufibacter tibetensis CCTCC AB 208084 T (94.4%). The DNA G+C content of strain THG-T16 T was determined to be 46.7 mol%. DNA–DNA hybridization values between strain THG-T16 T and N. koreensis KACC 16450 T , R. roseus KCTC 42217 T , R. immobilis CCTCC AB 2013351 T , R.tibetensis CCTCC AB 208084 T were 33.5 ± 0.5% (31.7 ± 0.7% reciprocal analysis), 28.1 ± 0.2% (25.2 ± 0.2%), 17.1 ± 0.9% (10.2 ± 0.6%) and 8.1 ± 0.3% (5.2 ± 0.1%). The polar lipids were identified as phosphatidylethanolamine, two unidentified aminophospholipids, an unidentified aminolipid and three unidentified lipids. The quinone was identified as MK-7 and the polyamine as sym -homospermidine. The major fatty acids were identified as C 16:1 ω 5 c , C 17:1 ω 6 c , iso -C 15:0 , summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ) and summed feature 4 ( iso -C 17:1 I and/or anteiso -C 17:1 B). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics, and DNA–DNA hybridization data, strain THG-T16 T represents a novel species of the genus Nibribacter , for which the name Nibribacter flagellatus sp. nov. is proposed. The type strain is THG-T16 T (= KACC 19188 T  = CCTCC AB 2016246 T ).