FEBS letters, 2018-04, Vol.592 (8), p.1400-1411
2018
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Details
- Autor(en) / Beteiligte
- Titel
- Gemin5 plays a role in unassembled‐U1 snRNA disposal in SMN‐deficient cells
- Ist Teil von
- FEBS letters, 2018-04, Vol.592 (8), p.1400-1411
- Ort / Verlag
- England
- Erscheinungsjahr
- 2018
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Gemin5 acts as a U1 small nuclear RNA (snRNA)‐binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein‐deficient conditions. We demonstrate that non‐Sm protein‐associated U1 snRNA and U1A are enriched in cytoplasmic granules and colocalize to P bodies in SMN‐deficient cells. Immunoprecipitation assays show increased associations of the U1 snRNP component U1A with P body components and Gemin5 in SMN‐deficient cells. More importantly, Gemin5 knockdown eliminates the unassembled U1 snRNP granules and rescues U1 snRNA levels in SMN‐deficient cells. Taken together, our study provides direct evidence that Gemin5 is involved in unassembled‐U1 snRNA disposal under conditions of SMN deficiency.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1002/1873-3468.13031Titel-ID: cdi_proquest_miscellaneous_2013788071
- Format
- –
- Schlagworte
- Cytoplasmic Granules - genetics, Cytoplasmic Granules - metabolism, Gemin5, HeLa Cells, Humans, Ribonucleoprotein, U1 Small Nuclear - genetics, Ribonucleoprotein, U1 Small Nuclear - metabolism, Ribonucleoproteins, Small Nuclear - genetics, Ribonucleoproteins, Small Nuclear - metabolism, RNA, Small Nuclear - genetics, RNA, Small Nuclear - metabolism, SMN deficiency, Survival of Motor Neuron 1 Protein - genetics, Survival of Motor Neuron 1 Protein - metabolism, Unassembled U1 snRNA disposal