Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Variations of the nirS-, nirK-, and nosZ-denitrifying bacterial communities in a northern Chinese soil as affected by different long-term irrigation regimes
Ist Teil von
Environmental science and pollution research international, 2018-05, Vol.25 (14), p.14057-14067
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2018
Quelle
SpringerLink
Beschreibungen/Notizen
Denitrification causes nitrogen loss from agricultural soils and emission of nitrous oxide (N
2
O). Water addition leads to an increase in soil moisture which greatly influenced soil denitrification. However, it is unclear how irrigation management affected the denitrifying bacterial communities in agricultural systems. In the present study, we investigated the abundance, diversity, and composition of the
nirS
-,
nirK
-, and
nosZ
-denitrifying bacterial communities in the soil under different long-term irrigation regimes by using real-time PCR (qPCR) and Illumina MiSeq sequencing approaches. Results showed that the abundance of
nosZ
gene was 3.94–6.01 and 35.09–60.21 times more than that of
nirS
and
nirK
genes, and the abundance of
nirS
gene was 5.84–15.30 times higher than that of
nirK
gene, respectively, in different irrigation treatments. However, the Alpha diversity indices of the
nirK
-denitrifying bacterial community were higher than those of the
nirS
- and
nosZ
-denitrifying bacterial communities. Proteobacteria was the predominant phylum for all the denitrifying bacterial communities, and significant differences were observed in relative abundance of Alphaproteobacteria and Betaproteobacteria in predominant class between different irrigation treatments for the
nirS
- and
nosZ
-denitrifying bacterial communities, respectively. Irrigation significantly affected the abundance, Shannon and Invsimpson indices, and structure of the
nirS
- and
nosZ
-denitrifying bacterial communities, whereas it only minor influenced the structure of the
nirK
-denitrifying bacterial community. Furthermore, the shifts in abundance, diversity, and structure of the
nirS
- and
nosZ
-denitrifying bacterial communities correlated significantly with the soil property variations; however, no soil property was significantly correlated with the abundance and Alpha diversity index of the
nirK
-denitrifying bacterial community. Our results demonstrate that different long-term irrigation regimes greatly altered the abundance, diversity, and structure of the
nirS
- and
nosZ
- rather than the
nirK
-denitrifying bacterial communities.