Bioconjugate chemistry, 2018-04, Vol.29 (4), p.1025-1029
2018
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Details
- Autor(en) / Beteiligte
- Titel
- Synergy of Two Highly Specific Biomolecular Recognition Events: Aligning an AT-Hook Peptide in DNA Minor Grooves via Covalent Conjugation to 2′-Amino-LNA
- Ist Teil von
- Bioconjugate chemistry, 2018-04, Vol.29 (4), p.1025-1029
- Ort / Verlag
- United States: American Chemical Society
- Erscheinungsjahr
- 2018
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- Two highly specific biomolecular recognition events, nucleic acid duplex hybridization and DNA-peptide recognition in the minor groove, were coalesced in a miniature ensemble for the first time by covalently attaching a natural AT-hook peptide motif to nucleic acid duplexes via a 2′-amino-LNA scaffold. A combination of molecular dynamics simulations and ultraviolet thermal denaturation studies revealed high sequence-specific affinity of the peptide–oligonucleotide conjugates (POCs) when binding to complementary DNA strands, leveraging the bioinformation encrypted in the minor groove of DNA duplexes. The significant cooperative DNA duplex stabilization may pave the way toward further development of POCs with enhanced affinity and selectivity toward target sequences carrying peptide-binding genetic islands.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1043-1802eISSN: 1520-4812DOI: 10.1021/acs.bioconjchem.8b00101Titel-ID: cdi_proquest_miscellaneous_2011273087
- Format
- –
- Schlagworte
- Affinity, Amino acids, Binding, Chemical bonds, Complementary DNA, Conjugation, Deoxyribonucleic acid, DNA, Gene sequencing, Grooves, Hybridization, Molecular biology, Molecular chains, Molecular dynamics, Next-generation sequencing, Nucleic acids, Oligonucleotides, Peptides, Recognition, Thermal denaturation