Journal of plant physiology, 2009-07, Vol.166 (11), p.1223-1227
2009
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Details
- Autor(en) / Beteiligte
- Titel
- Zn stress-induced inhibition of bean PvSR2– GUS fusion gene splicing is gene-specific in transgenic tobacco
- Ist Teil von
- Journal of plant physiology, 2009-07, Vol.166 (11), p.1223-1227
- Ort / Verlag
- Munich: Elsevier GmbH
- Erscheinungsjahr
- 2009
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- The stress-related gene no. 2 of Phaseolus vulgaris ( PvSR2) is metal inducible and contains a single intron. Here, we report that Zn stress inhibited the splicing of the PvSR2– β-glucuronidase ( GUS) fusion gene in a concentration- and time-dependent manner in tobacco seedlings. The inhibition appears to be specific for the PvSR2–GUS transgene: splicing of four endogenous tobacco genes was unaffected by Zn stress. Our results provide in vivo evidence that Zn stress-dependent intron retention is transgene specific in plants.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0176-1617eISSN: 1618-1328DOI: 10.1016/j.jplph.2009.01.010Titel-ID: cdi_proquest_miscellaneous_20111537
- Format
- –
- Schlagworte
- alternative splicing, Beans, beta-glucuronidase, Biological and medical sciences, cadmium, Fundamental and applied biological sciences. Psychology, Gene Expression Regulation, Plant - drug effects, Gene Expression Regulation, Plant - genetics, Gene Fusion - genetics, heavy metals, Intron retention, Intron splicing, introns, Introns - genetics, Nicotiana - drug effects, Nicotiana - genetics, Nicotiana tabacum, Phaseolus - genetics, Phaseolus vulgaris, Plant physiology and development, Plants, Genetically Modified - drug effects, Plants, Genetically Modified - genetics, PvSR2 intron, RNA Splicing - drug effects, seedlings, stress-related gene, tobacco, Transgene specific, transgenes, transgenic plants, zinc, Zinc - pharmacology