Details

Autor(en) / Beteiligte

• Okamoto, Tatsuro
• Takada, Kazuki
• Sato, Seijiro
• Toyokawa, Gouji
• Tagawa, Tetsuzo
• Shoji, Fumihiro
• Nakanishi, Ryota
• Oki, Eiji
• Koike, Terumoto
• Nagahashi, Masayuki
• Ichikawa, Hiroshi
• Shimada, Yoshifumi
• Watanabe, Satoshi
• Kikuchi, Toshiaki
• Akazawa, Kouhei
• Lyle, Stephen
• Takabe, Kazuaki
• Okuda, Shujiro
• Sugio, Kenji
• Wakai, Toshifumi
• Tsuchida, Masanori
• Maehara, Yoshihiko

Titel

Clinical and Genetic Implications of Mutation Burden in Squamous Cell Carcinoma of the Lung

Ist Teil von

• Annals of surgical oncology, 2018-06, Vol.25 (6), p.1564-1571

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Lung squamous cell carcinoma (LSCC) is a major histological subtype of lung cancer. In this study, we investigated genomic alterations in LSCC and evaluated the clinical implications of mutation burden (MB) in LSCC. Methods Genomic alterations were determined in Japanese patients with LSCC ( N  = 67) using next-generation sequencing of 415 known cancer genes. MB was defined as the number of non-synonymous mutations per 1 Mbp. Programmed death-ligand 1 (PD-L1) protein expression in cancer cells was evaluated by immunohistochemical analysis. Results TP53 gene mutations were the most common alteration ( n  = 51/67, 76.1%), followed by gene alterations in cyclin-dependent kinase inhibitor 2B ( CDKN2B ; 35.8%), CDKN2A (31.3%), phosphatase and tensin homolog (30.0%), and sex-determining region Y-box 2 ( SOX2 , 28.3%). Histological differentiation was significantly poorer in tumors with high MB (greater than or equal to the median MB) compared with that in tumors with low MB (less than the median MB; p = 0.0446). The high MB group had more tumors located in the upper or middle lobe than tumors located in the lower lobe ( p = 0.0019). Moreover, cancers in the upper or middle lobes had significantly higher MB than cancers in the lower lobes ( p = 0.0005), and tended to show higher PD-L1 protein expression ( p  = 0.0573). SOX2 and tyrosine kinase non-receptor 2 amplifications were associated with high MB ( p  = 0.0065 and p  = 0.0010, respectively). Conclusions The MB level differed according to the tumor location in LSCC, suggesting that the location of cancer development may influence the genomic background of the tumor.