Details

Autor(en) / Beteiligte

• Feng, Fang
• Zhou, Qingqing
• Yang, Yanfang
• Zhao, Fangkun
• Du, Renpeng
• Han, Ye
• Xiao, Huazhi
• Zhou, Zhijiang

Titel

Characterization of highly branched dextran produced by Leuconostoc citreum B-2 from pineapple fermented product

Ist Teil von

• International journal of biological macromolecules, 2018-07, Vol.113, p.45-50

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A strain Leuconostoc citreum B-2 was isolated from homemade fermentation product of pineapple and its polysaccharide yield was 28.3g/L after cultivating the strain in Man-Rogosa-Sharpe (MRS) medium with 75g/L sucrose. The exopolysaccharide (EPS) was characterized by gas chromatography (GC), Fourier-transform infrared (FT-IR) spectra, high-performance size-exclusion chromatography (HPSEC), nuclear magnetic resonance (NMR) spectroscopy and scanning electron microscope (SEM) analysis in present study. The monosaccharide composition of EPS was glucose and molecular weight was 3.77×106Da. FT-IR and NMR spectra revealed that the B-2 EPS was composed of 75% α-(1→6) linked d-glucopyranose units existing in the main chain with 19% α-(1→3) branching and only a few α-(1→2) branching. The SEM of the dried EPS appeared irregular sheets with glittering surface and compact structure. Water solubility index and water holding capacity of B-2 EPS were 80% and 450%, respectively. All the mentioned characteristics suggested that the EPS has a potential application in the food, cosmetic and pharmaceuticals industry. [Display omitted] •Leuconostoc citreum B-2 was isolated from homemade fermentation product of pineapple.•The polysaccharide yield was 28.3g/L when strain was cultivated in the MRS medium with 75g/L sucrose.•The EPS was composed of 75% α-(1→6) linked d-glucopyranose units with 19% α-(1→3) and only a few α-(1→2) linked branches.•The EPS was characterized by GC, FT-IR, HPSEC, NMR and SEM.•The EPS has a potential application in the food, cosmetic and pharmaceuticals industry.