Details

Autor(en) / Beteiligte

• Wani, Sajad Ahmad
• Sahu, Amit Ranjan
• Saxena, Shikha
• Rajak, Kaushal Kishor
• Saminathan, M.
• Sahoo, Aditya Prasad
• Kanchan, Sonam
• Pandey, Aruna
• Mishra, Bina
• Muthuchelvan, D.
• Tiwari, Ashok Kumar
• Mishra, Bishnu Prasad
• Singh, Raj Kumar
• Gandham, Ravi Kumar

Titel

Expression kinetics of ISG15, IRF3, IFNγ, IL10, IL2 and IL4 genes vis-a-vis virus shedding, tissue tropism and antibody dynamics in PPRV vaccinated, challenged, infected sheep and goats

Ist Teil von

• Microbial pathogenesis, 2018-04, Vol.117, p.206-218

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Here, we studied the in vivo expression of Th1 (IL2 and IFN gamma) and Th2 (IL4 and IL10) - cytokines and antiviral molecules - IRF3 and ISG15 in peripheral blood mononuclear cells in relation to antigen and antibody dynamics under Peste des petits ruminants virus (PPRV) vaccination, infection and challenge in both sheep and goats. Vaccinated goats were seropositive by 9 days post vaccination (dpv) while in sheep idiosyncratic response was observed between 9 and 14 dpv for different animals. Expression of PPRV N gene was not detected in PBMCs of vaccinated and vaccinated challenged groups of both species, but was detected in unvaccinated infected PBMCs at 9 and 14 days post infection. The higher viral load at 9 dpi coincided with the peak clinical signs of the disease. The peak in viral replication at 9 dpi correlated with significant expression of antiviral molecules IRF3, ISG15 and IFN gamma in both the species. With the progression of disease, the decrease in N gene expression also correlated with the decrease in expression of IRF3, ISG15 and IFN gamma. In the unvaccinated infected animals ISG15, IRF3, IFN gamma and IL10 expression was higher than vaccinated animals. The IFN gamma expression predominated over IL4 in both vaccinated and infected animals with the infected exhibiting a stronger Th1 response. The persistent upregulation of this antiviral molecular signature - ISG15 and IRF3 even after 2 weeks post vaccination, presumably reflects the ongoing stimulation of innate immune cells. •PPRV N gene expression detected only in PBMCs of unvaccinated infected animals.•Infected animals exhibited a stronger Th1 response than vaccinated.•ISG15, IRF3, IFNγ and IL10 expression was higher in infection than in vaccination.•Peak in viral replication correlated with expression of antiviral molecules.•ISG15 and IRF3 may play a major role in vaccine induced immune response.