Details

Autor(en) / Beteiligte

• Choi, Eun-Ju
• Oh, Hyun-Mee
• Na, Bo-Ra
• Ramesh, T. P.
• Lee, Hyun-Ju
• Choi, Chang-Soo
• Choi, Suck-Chei
• Oh, Tae-Young
• Choi, Suck-Jun
• Chae, Jeong-Ryong
• Kim, Sang-Wook
• Jun, Chang-Duk

Titel

Eupatilin Protects Gastric Epithelial Cells from Oxidative Damage and Down-Regulates Genes Responsible for the Cellular Oxidative Stress

Ist Teil von

• Pharmaceutical research, 2008-06, Vol.25 (6), p.1355-1364

Ort / Verlag

Boston: Springer US

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• Purpose The formulated ethanol extract (DA-9601) of Artemisia asiatica has pronounced anti-inflammatory activities and exhibits cytoprotective effects against gastrointestinal damage. Here we investigated whether eupatilin, a major component of DA-9601, has a property of antioxidant activity and protects gastric epithelial cells from H 2 O 2 -induced damage. Methods The protective effect of eupatilin against H 2 O 2 -induced damages was studied in gastric epithelial AGS cells by measuring wound healing, cell proliferation, and cell viability. Global gene expression profiling was obtained by high-density microarray. Results Hydrogen peroxide significantly delayed epithelial migration in wounded area. In contrast, eupatilin prevented the reduction of epithelial migration induced by H 2 O 2 . Eupatilin also ameliorated H 2 O 2 -induced actin disruption in AGS cells. Interestingly, treatment with eupatilin dramatically inhibited FeSO 4 -induced ROS production in a dose-dependent manner. In addition, eupatilin protected cells from FeSO 4 -induced F-actin disruption. With high-density microarray, we identified dozens of genes whose expressions were up-regulated in H 2 O 2 -treated cells. We found that eupatilin reduces the expression of such oxidative-responsible genes as HO-1, PLAUR and TNFRSF10A in H 2 O 2 -treated cells. Conclusion These results suggest that eupatilin acts as a novel antioxidant and may play an important role in DA-9601-mediated effective repair of the gastric mucosa.