Details

Autor(en) / Beteiligte

• Silva, Carlos D.S.
• Paz, Iury A.
• Abreu, Felipe D.
• de Sousa, Aurideia P.
• Veríssimo, Carla P.
• Nascimento, Nilberto R.F.
• Paulo, Tércio F.
• Zampieri, Davila
• Eberlin, Marcos N.
• Gondim, Ana C.S.
• Andrade, Loraine C.
• Carvalho, Idalina M.M.
• Sousa, Eduardo H.S.
• Lopes, Luiz G.F.

Titel

Thiocarbonyl-bound metallonitrosyl complexes with visible-light induced DNA cleavage and promising vasodilation activity

Ist Teil von

• Journal of inorganic biochemistry, 2018-05, Vol.182, p.83-91

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Nitric oxide has been involved in many key biological processes such as vasodilation, platelet aggregation, apoptosis, memory function, and this has drawn attention to the development of exogenous NO donors. Metallonitrosyl complexes are an important class of these compounds. Here, two new ruthenium nitrosyl complexes containing a thiocarbonyl ligand, with the formula cis-[Ru(phen)2(L)(NO)](PF6)3 (phen = phenantroline, L = thiourea or thiobenzamide), were synthesized and characterized by electronic spectroscopy, FTIR, NMR, mass spectrometry and voltammetric techniques. Theoretical calculations using Density Functional Theory (DFT) and Time-dependent Density Functional Theory (TD-DFT) were also used and further supported the characterizations of these complexes. An efficient release of nitric oxide by blue light was validated using a NO/HNO probe: 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, known as cPTIO. Interestingly, the complex containing thiourea cleaved DNA even in the dark, while both complexes showed great DNA photocleavage activity in blue light. This process might work mainly through NO and hydroxyl radical production. Additionally, these complexes showed promising vasodilator activity, whose mechanism of action was investigated using N-Nitro-l-arginine methyl ester hydrochloride (L-NAME) and 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and compared to sodium nitroprusside. Both compounds were indeed NO-mediated heme-dependent activators of soluble guanylate cyclase. Additionally, they did not show any significant cytotoxicity against cancer cell lines U87 and GBM02. Altogether, these results supported both complexes having potential pharmacological applications that deserve further studies. Nitrosyl ruthenium complexes containing thiocarbonyl-bound ligands release nitric oxide and promoted DNA damage under blue light. Vasodilation assay indicates these compounds are potential anti-hypertensive working on the soluble guanylate cyclase activation through heme. [Display omitted] •The complexes release nitric oxide photo-induced.•Ruthenium complexes showed promising vasodilation activity.•Ruthenium complexes showed cleave DNA under blue light irradiation.