Details

Autor(en) / Beteiligte

• Jiratchariyakul, Weena
• Vongsakul, Molvibha
• Sunthornsuk, Leena
• Moongkarndi, Primchanien
• Narintorn, Ammararat
• Somanabandhu, Aimon
• Okabe, Hikaru
• Frahm, August Wilhelm

Titel

Immunomodulatory effect and quantitation of a cytotoxic glycosphingolipid from Murdannia loriformis

Ist Teil von

• Journal of natural medicines, 2006-07, Vol.60 (3), p.210-216

Ort / Verlag

Japan

Erscheinungsjahr

2006

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• NMR signal reassignments for a cytotoxic glycosphingolipid compound, 2, β-O-D-glucopyranosyl-2-(2'-hydroxy-Z-6'-enecosamide)sphingosine, isolated from an ethanolic extract of the herb Murdannia loriformis, have been achieved by use of FAB-MS, and 1D and 2D H and C NMR. The amount of 2 in the herb juice was quantitatively determined by use of a validated HPLC method (RP-18, MeOH-H O, UV detection at 210 nm). The immunomodulatory effect of the herb juice and of 2 was proved by means of in vitro cellular immunological assays. Compound 2 at a concentration of 13 nmol L stimulated PBMC proliferation and increased the CD 3,4:CD 3,8 ratio in T lymphocytes.