Journal of bioscience and bioengineering, 2018-07, Vol.126 (1), p.9-14
2018
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- Autor(en) / Beteiligte
- Titel
- N-glycan sialylation in a silkworm-baculovirus expression system
- Ist Teil von
- Journal of bioscience and bioengineering, 2018-07, Vol.126 (1), p.9-14
- Ort / Verlag
- Japan: Elsevier B.V
- Erscheinungsjahr
- 2018
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- A silkworm-baculovirus system is particularly effective for producing recombinant proteins, including glycoproteins. However, N-glycan structures in silkworm differ from those in mammals. Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues. Sialic acid on N-glycans plays important roles in protein functions. Therefore, we developed pathways for galactosylation and sialylation in silkworm. Sialylated N-glycans on proteins were successfully produced in silkworm by co-expressing galactosyltransferase and sialyltransferase and providing an external supply of a sialylation-related substrate. α2,3/α2,6 Sialylation to N-glycans was controlled by changing the type of sialyltransferase expressed in silkworm. Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures. Our results provide new information on the control of N-glycosylation in silkworm. [Display omitted]
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1389-1723eISSN: 1347-4421DOI: 10.1016/j.jbiosc.2018.01.007Titel-ID: cdi_proquest_miscellaneous_2001910925
- Format
- –
- Schlagworte
- Animals, Baculoviridae - genetics, Baculoviridae - metabolism, Baculovirus, Bombyx - genetics, Bombyx - metabolism, Cells, Cultured, Cloning, Molecular, Expression, Galactose - metabolism, Galactosyltransferases - genetics, Galactosyltransferases - metabolism, Gene Expression, Genetic Vectors - genetics, Genetic Vectors - metabolism, Glycoproteins - biosynthesis, Glycoproteins - isolation & purification, Glycoproteins - metabolism, Glycosylation, Humans, Mannose - metabolism, N-Acetylglucosaminyltransferases - genetics, N-Acetylglucosaminyltransferases - metabolism, N-glycan, Polysaccharides - metabolism, Protein Engineering - methods, Protein Processing, Post-Translational - genetics, Recombinant Proteins - genetics, Recombinant Proteins - isolation & purification, Recombinant Proteins - metabolism, Sialylation, Sialyltransferases - genetics, Sialyltransferases - metabolism, Silkworm