FEMS microbiology ecology, 2007-10, Vol.62 (1), p.118-130
2007
Details
- Autor(en) / Beteiligte
- Titel
- Dominance of Geobacteraceae in BTX-degrading enrichments from an iron-reducing aquifer
- Ist Teil von
- FEMS microbiology ecology, 2007-10, Vol.62 (1), p.118-130
- Ort / Verlag
- Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
- Erscheinungsjahr
- 2007
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Microbial community structure was linked to degradation potential in benzene-, toluene- or xylene- (BTX) degrading, iron-reducing enrichments derived from an iron-reducing aquifer polluted with landfill leachate. Enrichments were characterized using 16S rRNA gene-based analysis, targeting of the benzylsuccinate synthase-encoding bssA gene and phospholipid fatty acid (PLFA) profiling in combination with tracking of labelled substrate. 16S rRNA gene analysis indicated the dominance of Geobacteraceae, and one phylotype in particular, in all enrichments inoculated with polluted aquifer material. Upon cultivation, progressively higher degradation rates with a concomitant decrease in species richness occurred in all primary incubations and successive enrichments. Yet, the same Geobacteraceae phylotype remained common and dominant, indicating its involvement in BTX degradation. However, the bssA gene sequences in BTX degrading enrichments differed considerably from those of Geobacter isolates, suggesting that the first steps of toluene, but also benzene and xylene oxidation, are carried out by another member of the enrichments. Therefore, BTX would be synthrophically degraded by a bacterial consortium in which Geobacteraceae utilized intermediate metabolites. PLFA analysis in combination with ¹³C-toluene indicated that the enriched Geobacteraceae were assimilating carbon originally present in toluene. Combined with previous studies, this research suggests that Geobacteraceae play a key role in the natural attenuation of each BTX compound in situ.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0168-6496eISSN: 1574-6941DOI: 10.1111/j.1574-6941.2007.00371.xTitel-ID: cdi_proquest_miscellaneous_19999378
- Format
- –
- Schlagworte
- Aquifers, Attenuation, Bacteria - chemistry, Bacteria - classification, Bacteria - genetics, Bacteria - isolation & purification, Benzene, Benzene - metabolism, Benzylsuccinate synthase, Biodegradation, Biodegradation of pollutants, Biodegradation, Environmental, Biodiversity, Biological and medical sciences, Biotechnology, BTX, Carbon 13, Carbon Isotopes - metabolism, Carbon-Carbon Lyases - genetics, Community structure, Consortia, Cultivation, Degradation, Deltaproteobacteria - chemistry, Deltaproteobacteria - classification, Deltaproteobacteria - genetics, Deltaproteobacteria - isolation & purification, Desulfuromonaceae, DNA, Bacterial - chemistry, DNA, Bacterial - genetics, DNA, Ribosomal - chemistry, DNA, Ribosomal - genetics, Dominance, Ecology, Enrichment, Environment and pollution, Fatty acids, Fatty Acids - analysis, Fundamental and applied biological sciences. Psychology, Gene sequencing, Geobacter, Geobacteraceae, Hydrocarbons, In situ leaching, Industrial applications and implications. Economical aspects, Iron, Iron - metabolism, iron-reducing, Isotope Labeling, Landfills, Leachates, Metabolites, Microbiology, Microorganisms, Molecular Sequence Data, Natural attenuation, Oxidation, Oxidation-Reduction, Phospholipids, Phospholipids - analysis, Pollution control, RNA, Ribosomal, 16S - genetics, rRNA 16S, Species richness, Substrates, Toluene, Toluene - metabolism, Waste disposal sites, Water Microbiology, Water Pollutants, Chemical - chemistry, Water Pollutants, Chemical - metabolism, Xylene, Xylenes - metabolism