Science (American Association for the Advancement of Science), 2018-01, Vol.359 (6371)
2018
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- Autor(en) / Beteiligte
- Titel
- Phase separation of a yeast prion protein promotes cellular fitness
- Ist Teil von
- Science (American Association for the Advancement of Science), 2018-01, Vol.359 (6371)
- Ort / Verlag
- United States: The American Association for the Advancement of Science
- Erscheinungsjahr
- 2018
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Despite the important role of prion domains in neurodegenerative disease, their physiological function has remained enigmatic. Previous work with yeast prions has defined prion domains as sequences that form self-propagating aggregates. Here, we uncovered an unexpected function of the canonical yeast prion protein Sup35. In stressed conditions, Sup35 formed protective gels via pH-regulated liquid-like phase separation followed by gelation. Phase separation was mediated by the N-terminal prion domain and regulated by the adjacent pH sensor domain. Phase separation promoted yeast cell survival by rescuing the essential Sup35 translation factor from stress-induced damage. Thus, prion-like domains represent conserved environmental stress sensors that facilitate rapid adaptation in unstable environments by modifying protein phase behavior.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.aao5654Titel-ID: cdi_proquest_miscellaneous_1989538005
- Format
- –
- Schlagworte
- Adaptation, Agglomeration, Aggregates, Amino acids, Catalysis, Catalytic Domain, Cell survival, Compartments, Condensates, Crosslinking, Environmental changes, Environmental conditions, Environmental stress, Fitness, Fungi, Gelation, Gels, Genetic analysis, Glutamine, Glycine, Guanosine, Guanosine triphosphatases, Heredity, Hydrogen ions, Hydrogen-Ion Concentration, Machinery and equipment, Neurodegenerative diseases, Neurological diseases, Peptide Termination Factors - chemistry, Peptide Termination Factors - metabolism, pH effects, pH sensors, Phase separation, Phase Transition, Phase transitions, Phenotypic variations, Physiochemistry, Physiology, Prion protein, Prion Proteins - chemistry, Prion Proteins - metabolism, Proteins, Reproductive fitness, Saccharomyces cerevisiae - cytology, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - chemistry, Saccharomyces cerevisiae Proteins - metabolism, Salts, Sensors, Sequences, Serine, Stress, Physiological, Stresses, Translation, Translation termination, Triphosphatase, Tyrosine, Yeast