Details

Autor(en) / Beteiligte

• Neuhaus, H.
• Van der Marel, M.
• Caspari, N.
• Meyer, W.
• Enss, M.-L.
• Steinhagen, D.

Titel

Biochemical and histochemical study on the intestinal mucosa of the common carp Cyprinus carpio L., with special consideration of mucin glycoproteins

Ist Teil von

• Journal of fish biology, 2007-05, Vol.70 (5), p.1523-1534

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2007

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• The biochemical and histochemical properties of intestinal mucin glycoproteins of virus and parasite‐free common carp Cyprinus carpio were investigated. The presence of carbohydrates in mucin glycoproteins could be demonstrated by histochemical methods, but generally, no obvious differences in specific staining for mucin glycoproteins were observed in contrast to biochemical techniques. Biochemical staining methods displayed differences in structure and composition of intestinal glycoproteins. Released intestinal glycoproteins contained two types of mucin glycoproteins: type 1 mucins displayed a size of >2000 kDa, and were highly glycosylated, while type 2 mucins ranged between 700 and 70 kDa, and were weakly glycosylated. In epithelial (intracellular) glycoproteins, mainly N‐acetyl‐α‐galactosamine and mannose were found, while in luminal (extracellular) glycoproteins in addition sialic acid was evident. Fucose was not detected. Thus, structure and composition of intestinal glycoproteins of common carp were similar to those found in mammals.