Details

Autor(en) / Beteiligte

• Liu, Xiaofeng
• Wang, Xiaoyu
• Wang, Qian
• Luo, Mingyang
• Guo, Huancheng
• Gong, Wenjie
• Tu, Changchun
• Sun, Jinfu

Titel

The eukaryotic translation initiation factor 3 subunit E binds to classical swine fever virus NS5A and facilitates viral replication

Ist Teil von

• Virology (New York, N.Y.), 2018-02, Vol.515, p.11-20

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Classical swine fever virus (CSFV) NS5A protein is a multifunctional protein, playing critical roles in viral RNA replication, translation and assembly. To further explore its functions in viral replication, interaction of NS5A with host factors was assayed using a his-tag “pull down” assay coupled with shotgun LC-MS/MS. Host protein translation initiation factor 3 subunit E was identified as a binding partner of NS5A, and confirmed by co-immunoprecipitation and co-localization analysis. Overexpression of eIF3E markedly enhanced CSFV genomic replication, viral protein expression and production of progeny virus, and downregulation of eIF3E by siRNA significantly decreased viral proliferation in PK-15 cells. Luciferase reporter assay showed an enhancement of translational activity of the internal ribosome entry site of CSFV by eIF3E and a decrease in cellular translation by NS5A. These data indicate that eIF3E plays an important role in CSFV replication, thereby identifying it as a potential target for inhibition of the virus. [Display omitted] •CSFV NS5A protein interacts with host protein translation initiation factor 3 subunit E (eIF3E).•eIF3E co-localizes with CSFV NS5A and viral dsRNA in cells.•eIF3E positively modulates CSFV replication.•eIF3E enhance CSFV IRES translational efficiency.•NS5A inhibit host translation.