Details

Autor(en) / Beteiligte

• Gao, Jian
• Ko, Tzu‐Ping
• Chen, Lu
• Malwal, Satish R.
• Zhang, Jianan
• Hu, Xiangying
• Qu, Fiona
• Liu, Weidong
• Huang, Jian‐Wen
• Cheng, Ya‐Shan
• Chen, Chun‐Chi
• Yang, Yunyun
• Zhang, Yonghui
• Oldfield, Eric
• Guo, Rey‐Ting

Titel

“Head‐to‐Middle” and “Head‐to‐Tail” cis‐Prenyl Transferases: Structure of Isosesquilavandulyl Diphosphate Synthase

Ist Teil von

• Angewandte Chemie International Edition, 2018-01, Vol.57 (3), p.683-687

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We report the first X‐ray crystallographic structure of the “head‐to‐middle” prenyltransferase, isosesquilavandulyl diphosphate synthase, involved in biosynthesis of the merochlorin class of antibiotics. The protein adopts the ζ or cis‐prenyl transferase fold but remarkably, unlike tuberculosinol adenosine synthase and other cis‐prenyl transferases (e.g. cis‐farnesyl, decaprenyl, undecaprenyl diphosphate synthases), the large, hydrophobic side chain does not occupy a central hydrophobic tunnel. Instead, it occupies a surface pocket oriented at 90° to the hydrophobic tunnel. Product chain‐length control is achieved by squeezing out the ligand from the conventional allylic S1 binding site, with proton ion being achieved using a diphosphate‐Asn‐Ser relay. The structures revise and unify our thinking as to the mechanism of action of many other prenyl transferases and may also be of use in engineering new merochlorin‐class antibiotics. Enzyme catalysis: Structures of a novel “head‐to‐middle” isoprenoid biosynthesis enzyme reveal a new mechanism of action for many enzymes involved in isoprenoid biosynthesis. This mechanism involves proton ion through phosphate groups and a proton relay.