Details

Autor(en) / Beteiligte

• Zhang, Yu
• Xiang, Yunlong
• Yin, Qiangzong
• Du, Zhenhai
• Peng, Xu
• Wang, Qiujun
• Fidalgo, Miguel
• Xia, Weikun
• Li, Yuanyuan
• Zhao, Zhen-ao
• Zhang, Wenhao
• Ma, Jing
• Xu, Feng
• Wang, Jianlong
• Li, Lei
• Xie, Wei

Titel

Dynamic epigenomic landscapes during early lineage specification in mouse embryos

Ist Teil von

• Nature genetics, 2018-01, Vol.50 (1), p.96-105

Ort / Verlag

New York: Nature Publishing Group US

Erscheinungsjahr

2018

Beschreibungen/Notizen

• In mammals, all somatic development originates from lineage segregation in early embryos. However, the dynamics of transcriptomes and epigenomes acting in concert with initial cell fate commitment remains poorly characterized. Here we report a comprehensive investigation of transcriptomes and base-resolution methylomes for early lineages in peri- and postimplantation mouse embryos. We found allele-specific and lineage-specific de novo methylation at CG and CH sites that led to differential methylation between embryonic and extraembryonic lineages at promoters of lineage regulators, gene bodies, and DNA-methylation valleys. By using Hi-C experiments to define chromatin architecture across the same developmental period, we demonstrated that both global demethylation and remethylation in early development correlate with chromatin compartments. Dynamic local methylation was evident during gastrulation, which enabled the identification of putative regulatory elements. Finally, we found that de novo methylation patterning does not strictly require implantation. These data reveal dynamic transcriptomes, DNA methylomes, and 3D chromatin landscapes during the earliest stages of mammalian lineage specification. Transcriptome, DNA methylome and Hi-C profiling of peri- and post-implantation mouse cell lineages identified allele- and lineage-specific methylation patterns. Global demethylation and remethylation correlate with megabase chromatin compartments.