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Analysis of the mechanism regulating the stability of rat macrophage inflammatory protein-2 mRNA in RBL-2H3 cells
Journal of cellular biochemistry, 2003-12, Vol.90 (5), p.976-986
Numahata, Keiichi
Komagata, Tatsuya
Hirasawa, Noriyasu
Someya, Koh-ichiro
Xiao, Yi-Qun
Ohuchi, Kazuo
2003
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Numahata, Keiichi
Komagata, Tatsuya
Hirasawa, Noriyasu
Someya, Koh-ichiro
Xiao, Yi-Qun
Ohuchi, Kazuo
Titel
Analysis of the mechanism regulating the stability of rat macrophage inflammatory protein-2 mRNA in RBL-2H3 cells
Ist Teil von
Journal of cellular biochemistry, 2003-12, Vol.90 (5), p.976-986
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2003
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
Using rat peritoneal neutrophils, the complete nucleotide sequence of rat macrophage inflammatory protein‐2 (MIP‐2) mRNA including 5′untranslated region (UTR) and 3′UTR was determined (GenBank Accession number, AB060092). It was found that the MIP‐2 mRNA has a 70 bp 5′UTR, a 303 bp coding region and a 728 bp 3′UTR which contains adenylate/uridylate (AU)‐rich areas defined as AU‐rich elements (AREs). Site‐directed mutagenesis studies using the tetracycline‐sensitive transactivator protein‐expressing rat basophilic leukemia cells (RBL‐2H3‐TO cells) revealed that MIP‐2 mRNA mutants which lack the 3′UTR are more stable than MIP‐2‐wild‐type (wt) mRNA. A MIP‐2 mRNA mutant in which some mutations were introduced to the ARE was also stable. The stability of MIP‐2 mRNA was low in untreated RBL‐2H3‐TO cells, but it increased in the antigen‐stimulated immunoglobulin E (IgE)‐sensitized cells. The antigen‐induced MIP‐2 mRNA stabilization was counteracted by the highly specific p38 mitogen‐activated protein kinase (MAPK) inhibitor SB203580 and the MAPK/ERK kinase (MEK‐1) inhibitor PD98059. These findings indicate that ARE is the cis‐element which mediates the rapid decay of MIP‐2 mRNA, and the antigen stimulation stabilizes MIP‐2 mRNA and the p38 MAPK and p44/42 MAPK pathways are involved in the antigen‐induced stabilization of MIP‐2 mRNA. © 2003 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0730-2312
eISSN: 1097-4644
DOI: 10.1002/jcb.10710
Titel-ID: cdi_proquest_miscellaneous_19724845
Format
–
Schlagworte
3' Untranslated Regions - genetics
,
5' Untranslated Regions - genetics
,
Animals
,
AU-rich element
,
Base Sequence
,
Chemokine CXCL2
,
Enzyme Inhibitors - pharmacology
,
Leukemia - genetics
,
Leukemia - metabolism
,
Leukemia - pathology
,
Male
,
MAP Kinase Kinase 1
,
MIP-2
,
Mitogen-Activated Protein Kinase 1 - metabolism
,
Mitogen-Activated Protein Kinase 3
,
Mitogen-Activated Protein Kinase Kinases - antagonists & inhibitors
,
Mitogen-Activated Protein Kinase Kinases - metabolism
,
Mitogen-Activated Protein Kinases - antagonists & inhibitors
,
Mitogen-Activated Protein Kinases - metabolism
,
Molecular Sequence Data
,
Monokines - genetics
,
Monokines - metabolism
,
mRNA stability
,
Mutagenesis, Site-Directed
,
Neutrophils - drug effects
,
Neutrophils - enzymology
,
p38 MAPK
,
p38 Mitogen-Activated Protein Kinases
,
p44/42 MAPK
,
Peritoneum - cytology
,
Rats
,
Rats, Sprague-Dawley
,
RBL-2H3 cells
,
Regulatory Sequences, Nucleic Acid - genetics
,
RNA Stability
,
RNA, Messenger - metabolism
,
Signal Transduction
,
Tumor Cells, Cultured
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