Details

Autor(en) / Beteiligte

• Pyle, Jesse D.
• Scholthof, Karen-Beth G.

Titel

De novo generation of helper virus-satellite chimera RNAs results in disease attenuation and satellite sequence acquisition in a host-dependent manner

Ist Teil von

• Virology (New York, N.Y.), 2018-01, Vol.514, p.182-191

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Panicum mosaic virus (PMV) is a helper RNA virus for satellite RNAs (satRNAs) and a satellite virus (SPMV). Here, we describe modifications that occur at the 3′-end of a satRNA of PMV, satS. Co-infections of PMV+satS result in attenuation of the disease symptoms induced by PMV alone in Brachypodium distachyon and proso millet. The 375 nt satS acquires ~100–200 nts from the 3′-end of PMV during infection and is associated with decreased abundance of the PMV RNA and capsid protein in millet. PMV-satS chimera RNAs were isolated from native infections of St. Augustinegrass and switchgrass. Phylogenetic analyses revealed that the chimeric RNAs clustered according to the host species from which they were isolated. Additionally, the chimera satRNAs acquired non-viral "linker" sequences in a host-specific manner. These results highlight the dynamic regulation of viral pathogenicity by satellites, and the selective host-dependent, sequence-based pressures for driving satRNA generation and genome compositions. [Display omitted] •Panicum mosaic virus (PMV) supports the systemic accumulation of a satRNA, satS.•satS attenuates the PMV-induced disease phenotype in multiple host grasses.•satS interferes with the PMV RNA and CP accumulation in a host-dependent manner.•The genomic RNAs of PMV and satS recombine to generate satS-PMV chimera RNAs.•The satS-PMV RNAs are present in natural infections of bioenergy and turf grasses.